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kinase domains ephb1  (Addgene inc)


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    Structured Review

    Addgene inc kinase domains ephb1
    Kinase Domains Ephb1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ephb1+kinase+domain/EPHB1_HUMAN_D0+(Plasmid+%2379694)/pmc12521615-172-6-10
    Average 93 stars, based on 4 article reviews
    kinase domains ephb1 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: High-throughput profiling of sequence recognition by tyrosine kinases and SH2 domains using bacterial peptide display
    Article Snippet: Recombinant DNA reagent , pET-His6-TEV-FGFR3(KD) , PMID: 30004690 , Addgene: 79731 , bacterial expression vector encoding the human FGFR3 kinase domain (residues 449–759) with an N-terminal His6-tag and TEV protease recognition sequence. .. Recombinant DNA reagent , pET-His6-TEV-EPHB1(KD) , PMID: 30004690 , Addgene: 79694 , bacterial expression vector encoding the human EPHB1 kinase domain (residues 602–896) with an N-terminal His6-tag and TEV protease recognition sequence. .. Recombinant DNA reagent , pET-His6-TEV-EPHB2(KD) , PMID: 30004690 , Addgene: 79697 , bacterial expression vector encoding the human EPHB2 kinase domain (residues 604–898) with an N-terminal His6-tag and TEV protease recognition sequence.

    Expressing:

    Article Title: High-throughput profiling of sequence recognition by tyrosine kinases and SH2 domains using bacterial peptide display
    Article Snippet: Recombinant DNA reagent , pET-His6-TEV-FGFR3(KD) , PMID: 30004690 , Addgene: 79731 , bacterial expression vector encoding the human FGFR3 kinase domain (residues 449–759) with an N-terminal His6-tag and TEV protease recognition sequence. .. Recombinant DNA reagent , pET-His6-TEV-EPHB1(KD) , PMID: 30004690 , Addgene: 79694 , bacterial expression vector encoding the human EPHB1 kinase domain (residues 602–896) with an N-terminal His6-tag and TEV protease recognition sequence. .. Recombinant DNA reagent , pET-His6-TEV-EPHB2(KD) , PMID: 30004690 , Addgene: 79697 , bacterial expression vector encoding the human EPHB2 kinase domain (residues 604–898) with an N-terminal His6-tag and TEV protease recognition sequence.

    Sequencing:

    Article Title: High-throughput profiling of sequence recognition by tyrosine kinases and SH2 domains using bacterial peptide display
    Article Snippet: Recombinant DNA reagent , pET-His6-TEV-FGFR3(KD) , PMID: 30004690 , Addgene: 79731 , bacterial expression vector encoding the human FGFR3 kinase domain (residues 449–759) with an N-terminal His6-tag and TEV protease recognition sequence. .. Recombinant DNA reagent , pET-His6-TEV-EPHB1(KD) , PMID: 30004690 , Addgene: 79694 , bacterial expression vector encoding the human EPHB1 kinase domain (residues 602–896) with an N-terminal His6-tag and TEV protease recognition sequence. .. Recombinant DNA reagent , pET-His6-TEV-EPHB2(KD) , PMID: 30004690 , Addgene: 79697 , bacterial expression vector encoding the human EPHB2 kinase domain (residues 604–898) with an N-terminal His6-tag and TEV protease recognition sequence.



    Similar Products

    kinase domains ephb1  (Addgene inc)
    93
    Addgene inc kinase domains ephb1
    Kinase Domains Ephb1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ephb1+kinase+domain/EPHB1_HUMAN_D0+(Plasmid+%2379694)/pmc12521615-172-6-10
    Average 93 stars, based on 1 article reviews
    kinase domains ephb1 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    human ephb1 kinase domain  (Addgene inc)
    90
    Addgene inc human ephb1 kinase domain
    ( A ) Heatmaps depicting the specificities of c-Abl, Fer, <t>EPHB1,</t> and EPHB2. Enrichment scores were log 2 -transformed and are displayed on a color scale from blue (disfavored sequence features, negative value), to white (neutral sequence features, near zero value), to red (favored sequence features, positive value). Values in the heatmaps are the average of three replicates. ( B ) Sequences of consensus peptides identified through X 5 -Y-X 5 screens, compared with previously reported SrcTide and AblTide sequences. ( C ) Phosphorylation kinetics of five consensus peptides against five kinases. Initial rates were normalized to the rate of the cognate consensus peptide. All peptides were used at a concentration of 100 μM, and the kinases were used at a concentration of 10–50 nM. Error bars represent the standard deviation from at least three measurements. Figure 2—source data 1. Position-specific amino acid enrichment matrices from the tyrosine kinase X 5 -Y-X 5 library screens. Matrices calculated with and without inclusion of multi-tyrosine sequences are provided.
    Human Ephb1 Kinase Domain, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ephb1+kinase+domain/pGL4%2E23+C2_13+(Plasmid+%2360289)/pmc10065799-31-17-9
    Average 90 stars, based on 1 article reviews
    human ephb1 kinase domain - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Heatmaps depicting the specificities of c-Abl, Fer, EPHB1, and EPHB2. Enrichment scores were log 2 -transformed and are displayed on a color scale from blue (disfavored sequence features, negative value), to white (neutral sequence features, near zero value), to red (favored sequence features, positive value). Values in the heatmaps are the average of three replicates. ( B ) Sequences of consensus peptides identified through X 5 -Y-X 5 screens, compared with previously reported SrcTide and AblTide sequences. ( C ) Phosphorylation kinetics of five consensus peptides against five kinases. Initial rates were normalized to the rate of the cognate consensus peptide. All peptides were used at a concentration of 100 μM, and the kinases were used at a concentration of 10–50 nM. Error bars represent the standard deviation from at least three measurements. Figure 2—source data 1. Position-specific amino acid enrichment matrices from the tyrosine kinase X 5 -Y-X 5 library screens. Matrices calculated with and without inclusion of multi-tyrosine sequences are provided.

    Journal: eLife

    Article Title: High-throughput profiling of sequence recognition by tyrosine kinases and SH2 domains using bacterial peptide display

    doi: 10.7554/eLife.82345

    Figure Lengend Snippet: ( A ) Heatmaps depicting the specificities of c-Abl, Fer, EPHB1, and EPHB2. Enrichment scores were log 2 -transformed and are displayed on a color scale from blue (disfavored sequence features, negative value), to white (neutral sequence features, near zero value), to red (favored sequence features, positive value). Values in the heatmaps are the average of three replicates. ( B ) Sequences of consensus peptides identified through X 5 -Y-X 5 screens, compared with previously reported SrcTide and AblTide sequences. ( C ) Phosphorylation kinetics of five consensus peptides against five kinases. Initial rates were normalized to the rate of the cognate consensus peptide. All peptides were used at a concentration of 100 μM, and the kinases were used at a concentration of 10–50 nM. Error bars represent the standard deviation from at least three measurements. Figure 2—source data 1. Position-specific amino acid enrichment matrices from the tyrosine kinase X 5 -Y-X 5 library screens. Matrices calculated with and without inclusion of multi-tyrosine sequences are provided.

    Article Snippet: Recombinant DNA reagent , pET-His6-TEV-EPHB1(KD) , PMID: 30004690 , Addgene: 79694 , bacterial expression vector encoding the human EPHB1 kinase domain (residues 602–896) with an N-terminal His6-tag and TEV protease recognition sequence.

    Techniques: Transformation Assay, Sequencing, Concentration Assay, Standard Deviation

    Cells displaying the X 5 -Y-X 5 library were treated with a kinase cocktail containing c-Src, c-Abl, AncSZ, and EPHB1 for 3 hr, then labeled with a pan-phosphotyrosine antibody (PY20 PerCP-eFluor 710) and analyzed by flow cytometry.

    Journal: eLife

    Article Title: High-throughput profiling of sequence recognition by tyrosine kinases and SH2 domains using bacterial peptide display

    doi: 10.7554/eLife.82345

    Figure Lengend Snippet: Cells displaying the X 5 -Y-X 5 library were treated with a kinase cocktail containing c-Src, c-Abl, AncSZ, and EPHB1 for 3 hr, then labeled with a pan-phosphotyrosine antibody (PY20 PerCP-eFluor 710) and analyzed by flow cytometry.

    Article Snippet: Recombinant DNA reagent , pET-His6-TEV-EPHB1(KD) , PMID: 30004690 , Addgene: 79694 , bacterial expression vector encoding the human EPHB1 kinase domain (residues 602–896) with an N-terminal His6-tag and TEV protease recognition sequence.

    Techniques: Labeling, Flow Cytometry

    Journal: eLife

    Article Title: High-throughput profiling of sequence recognition by tyrosine kinases and SH2 domains using bacterial peptide display

    doi: 10.7554/eLife.82345

    Figure Lengend Snippet:

    Article Snippet: Recombinant DNA reagent , pET-His6-TEV-EPHB1(KD) , PMID: 30004690 , Addgene: 79694 , bacterial expression vector encoding the human EPHB1 kinase domain (residues 602–896) with an N-terminal His6-tag and TEV protease recognition sequence.

    Techniques: Clone Assay, Over Expression, Strep-tag, Recombinant, Plasmid Preparation, Sequencing, Variant Assay, Expressing, Mutagenesis, Marker, Amplification, Synthesized, Western Blot, Purification, Software